Cimini Lab : Metaphase-to-anaphases

Metaphase-to-anaphases

The movie shows a mitotic PtK1 cell microinjected with X-rhodamine-labeled tubulin and Alexa 488-labeled CENP-F
antibodies, to fluorescently label kinetochore fibers (red) and kinetochores/spindle poles (green), respectively.

The movie starts in metaphase and progresses through anaphase. Initially, sister kinetochores oscillate back
and forth about the metaphase plate, as their respective kinetochore fibers lengthen and shorten.
As the cell enters anaphase and the sister chromatids separate, sister kinetochores move away from each other
as their kinetochore fibers shorten (anaphase A). Almost simultaneously, the two spindle poles move away from
each other leading to elongation of the mitotic spindle (anaphase B).

Digital images were acquired every 20-60 seconds at a 60X magnification. Elapsed time is shown in min:sec