Mitotic PtK1 cell microinjected with X-rhodamine-labeled tubulin and Alexa 488-labeled CENP-F antibodies, to fluorescently label kinetochore fibers (red) and kinetochores/spindle poles (green), respectively.
This cell possesses a merotelically oriented kinetochore that becomes hyperstretched as the cell enters anaphase and the kinetochore is pulled in opposite directions.

Digital images were acquired every 20-60 seconds at a 60X magnification. Elapsed time is shown in min:sec