The video shows the overlay of phase contrast and fluorescence microscopy images of a PtK1 cell expressing fluorescently-labeled ?-tubulin (red) to visualize centrosomes.
The cell undergoes mitosis and displays an anaphase lagging chromosome.

Phase contrast and fluorescence images were acquired almost simultaneously every 2 minutes, using a 4X4 binning. Each frame is played for 1/10th of a second. Elapsed time is displayed in hh:mm:ss